摘要
用预活化聚酰胺膜固定酪氨酸酶，制得性能良好的酪氨酸酶生物传感器，以此测定ＣＮ~－抑制剂的含量。在磷酸盐缓冲溶液中，以苯酚作底物，浓度范围为５×１０^（－６）～４×１０^（－５）ｍｏｌ／Ｌ，当苯酚浓度一定时，还原电流（－０．２００Ｖ，ｖｓ，ＳＣＥ）的下降值与ＣＮ~－浓度的平方根成线性关系，检测范围为５×１０^（－７）～２×１０^（－４）ｍｏｌ／Ｌ，有良好的选择性和重现性。
Cyandide inhibitor of tyrosinase was determied with biosensor of tyrosinase by using tyrosinase immobilized on a preactivated polyaminde membrane.Phenol with the range of concentration 5×10￣(-6)～4×10￣(-5) mol/L was chosen as substrate in phosphate buffer.The de-crease of substrate response have linear relation with square root of concentration of CN￣-with detection range of 5 ×10￣(-7)～2×10￣(-4) mol/L. The fine selectivity and reproducibility were obtained.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1995年第3期359-362,共4页
Chemical Journal of Chinese Universities
基金
江苏省教育委员会自然科学基金
