摘要
新皮啡肽Ⅰ、Ⅱ类似物的设计与合成胡晓愚，王锐，嘉庆，姬虹（兰州大学应用有机化学国家重点实验室、生物系↑，兰州，７３００００）关键词新皮啡肽，固相多肽合成，类阿片肽，类似物的设计内源性类阿片肽（Ｅｎｄｏｇｅｎｏｕｓｏｐｉｏｉｄｓ）是指由机体产生的具有与...
Endogenous opioid deltorphinⅠ (DEL Ⅰ),Ⅱ(DEL Ⅰ)and their five analogues ([L-Ala2]DEL Ⅰ,[L-Ala2]DEL Ⅱ,[Val4,Asp5]DEL Ⅰ,[Val4,Asp6]DEL Ⅰ,[Gly4,Asp7]DEL Ⅰ)were designed on a rational basis and were synthesized by solid phase peptide synthesis method.The synthesis was performed manually on BHA resin prepared by ourselves,DCC was used as coupling reagent and TFA as deboc reagent. Peptide-resin was cleavaged by HF, the crude peptides were obtained after lyophylization. The crude peptides were desalted on a sephadex Glo column, then the pure peptides were obtained by partitional chromatography on a sephadex G25 column. The purities of all synthetic peptides were greater than 95%, which were acquired by reversed HPLC analysis. The data we got from FABMS,AAA and sequence analysis were in agreement with the caculated values.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1995年第10期1569-1571,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
国家教委优秀年轻教师基金
博士点专项基金
关键词
新皮啡肽
多肽
类阿片肽
药物设计
肽
Deltorphins, Solid phase peptide synthesis, Opioid, Design of analogues
