摘要
采自离体繁殖枝条的悬钩子叶片能够在 MS 培养基上有效地分化出幼芽,分化率达79.17%。最佳植物生长调节剂组合是1.0mg·L^(-1)BAP 和0.1mg·L^(-1)IBA。在这一条件下,持续黑暗大大提高了分化率。叶片上的刻伤提高了分化率,但叶片切块却没有分化出芽。叶片在培养基上放置的方向对分化率没有影响。在同样培养基上,悬钩子茎段(无芽)也分化出幼芽,分化率达54.17%。叶柄难以再生,平均分化率仅有4.17%。
Blackberry leaves from in vitro propagated shoots have been effectively regenerated on MS medium,regeneration percentage reaching 79.17%.The best plant growth regulator combination was 1.0mg·L^(-1)BAP and 0.1mg·L^(-1)IBA,at which continuous darkness greatly improved the regeneration. Wounding enhanced regeneration percentage,but leaf segments did not regenerate.The orientation of blackberry leaves had no effect on the regeneration.
关键词
悬钩子
叶片
茎段
叶柄
离体培养
植株再生
黑莓
blackberry
leaf
stem segment
petiole
in vitro culture
regeneration
