摘要
为研究缺氧对肺动脉内皮细胞(PAEC)的影响,利用猪PAEC体外培养的方法,观察了缺氧时PAECⅧR:Ag阳性率、阳性PAEC荧光强度以及培养液中PGI_2含量的变化.结果表明,缺氧过程中,PAEC的ⅧR:Ag阳性率比对照组有明显降低,而阳性细胞的ⅧR:Ag荧光强度却比对照组明显增加.PAEC合成和分泌PGI_2的水平在缺氧开始时有所增加,但随着缺氧时间的延长却比对照组明显降低.PAEC在缺氧过程中的这些变化可能在缺氧性肺动脉高压和肺水肿的发生中发挥一定作用.
In this study, the effects of hypoxia on pulmonary arterial endothelial cells (PAEC) were investigated by using cultured pig's PAEC as a model. The changes of the rates of PAEC with positive immunofluorescence for factor Ⅷ related antigen ⅧR:Ag) ,the mean intensity of immunofluorescence in positive PAEC and the concentration of prostacylin (PGI2 ) in cultured media released by PAEC during hypoxia were observed. The hypoxia exposed PAEC demonstrated a decreased rate of positive im-munofluorescensce for ⅧR: Ag as compared with control, while an increased mean intensity of positive immunofluorscence was found. Concentrations of PGI2 in the media showed an initial increase and followed by a significant decrease as compared with control during the resting period of hypoxia. These results indicated that PAEC might play an important role in hypoxic pulmonary arterial hypertension and hypoxic pulmonary edema.
出处
《航天医学与医学工程》
CAS
CSCD
1995年第4期250-252,共3页
Space Medicine & Medical Engineering
基金
中科院低氧开放实验室资助课题
关键词
缺氧
肺动脉
因子Ⅷ
前列腺素
X类
内皮
hypoxia pulmonary artery factor Ⅷ prostaglandins x endothelium
