摘要
在建立人主动脉平滑肌细胞（ＳＭＣ）体外培养方法的基础上，观察了各种天然和氧化修饰型脂蛋白对培养人ＳＭＣ形态以及表型的影响。在氧化修饰低密度脂蛋白（ＯＸ－ＬＤＬ），氧化修饰高密度脂蛋白（Ｏｘ－ＨＤＬ）和氧化修饰极低密度脂蛋白（ＯＸ－ＶＬＤＬ）的作用下，ＳＭＣ形态发生以下变化：胞体变大，由梭形变为不规则多边形，突起细而长，而且“谷”、“峰”样生长方式也发生改变；ＬＤＬ和ｖＬＤＬ对ＳＭＣ的形态亦有影响，但不如氧化修饰脂蛋白明显；ＨＤＬ则无影响。电镜下观察，在ＯＸ－ＬＤＬ的诱导下，ＳＭＣ胞浆中的肌丝较对照组明显减少，而高氏器，内质网和线粒体则明显增多。上述结果说明，ＬＤＬ、ＶＬＤＬ、ＯＸ－ＬＤＬ、ＯＸ－ＶＬＤＬ和ＯＸ－ＨＤＬ的致动脉粥样硬化作用可能与刺激ＳＭＣ表型由“收缩”型向“合成”型转化有关。
The arterial smooth muscle cells(SMC) are the prdominant type of cells in atheroscle-rotic lesions, and their proliferation plays an importantrole in the process of AS genesis,We estabiished theprimary culture and sub-culture method for human ar-terial SMC and observed the effects LDL,VLDL,HDL,OX-LDL,OX-VLDL and OX-HDL on the mor-phological appearance and phenotype of cultured hu-man arterial SMC.The results revealed that the cellscultured in the medium containing OX-LDL,OX-VLDL and OX-HDL(25μg/ml ) became more irregularwith long finger-like projects and lacked a bipolarshape,and the characteristic.“hills” and “valleys” dis-appeared. LDL and vLDL also had the influence butin smaller degrees. HDL did not have any effect.TheSMC cultured in the presence of OX-LDL (25μg/ml )had less myofilaments and more endoplasmic reticu-lua, Golgi and mitochondria in cytoplasm comparedwith the cells in control group under electronmi-croscope. These results suggest that the atherogenicrole of LDL ,OX-LDL,OX-HDL,and OX-VLDL are closely related to their stimulating effectson the modulation of phenotype of arterial SMCs from“contractile”towards“synthetic”.
出处
《华西医科大学学报》
CSCD
1995年第2期146-150,共5页
Journal of West China University of Medical Sciences
基金
国家教委博士点专项基金
关键词
氧化修饰
脂蛋白
动脉平滑肌细胞
动脉粥样硬化
Lipoprotein Oxidation-modified lipoprotein Arterial smooth muscle cell phenotype Atherosclerosis
