摘要
在培养的鸡胚心室肌细胞上，我们采用膜片钳技术的细胞贴附式构型，研究了黄连素（Ｂｅｒｂｅｒｉｎｅ，ＢＲ）对细胞膜钙单通道电流的影响。结果表明：ＢＲ可使心室肌细胞膜钙单通道电流明显增加；钙通道可利用率增加１１４．２％（Ｐ＜０．００１）；钙通道开放概率增加７５．３％（Ｐ＜０．０５）。用心得安阻断β受体或用异搏定阻断钙通道，均可逆转ＢＲ的上述作用。以上提示：①ＢＲ是一种钙通道激活剂；②ＢＲ对钙通道的调节作用依赖于β受体介导的磷酸化过程。
On cultured embryonic chick ventricu-lar myocytes, we investigated the influence of berber-ine(BR)on single Ca￣(2+) channel current using patchclamp technique with cell-attached configuration. Theresults showed that BR could increase the single Ca￣(2+)channel current significantly and the increases of Openavailability(Ps)and open probability(Po)caused byBRwere 114.2%(P<0.001)and 75.3%(P<0.05),respectively. Theβ-receptor antagonist pro-pranolol and Ca￣(2+) channel blocker ispotin could reversethe effects of BR on single Ca￣(2+) channel, suggestingthat(1)BR works as a Ca￣(2+) channel agonist; (2)theregulating effects of BR on single Ca￣(2+) channel is de-pending upon the phosphorylation process induced byAreceptor activity.
出处
《华西医科大学学报》
CSCD
1995年第3期287-290,共4页
Journal of West China University of Medical Sciences
关键词
黄连素
心室肌细胞
钙电流
膜片钳
Berberine Cultured ventricular myocytes Ca￣(2+) channel current Patch clamp
