摘要
本文应用付立叶交换拉曼光谱技术测定了正常鼠晶状体和半乳糖诱发糖性白内障鼠晶状体激光拉曼光谱。并将两种谱结果相比较。这些谱表明，随着半乳糖诱发糖性白内障的形成，主要表现在８４０ｃｍ－１附近的酪氨酸残基双峰强度比明显不同。说明由于晶状体浑浊时，某些酪氨酸残基氢键强度随着形成大分子量蛋白质聚集体而增加。
AbstractThe Fourier Transform Raman spectroscopic method was employed to determine the contents of the normol rat lens and galactose-induced diabetic cateractous rat lens. Raman spectrum of disbetic cataractous lens was compared with that of the normal lens. These spectra show appreciable difference in the relative intensity of the tyrosine doublet near 840cm-1 during the galactose-induced diabetic cataract formation. This indicates that the strength of hydrogen bonding by certain tyrosyl residues enhances upon the aggregation of crystallines in the lens opacification.
