摘要
提出了一种新的酶固定化方法,即通过甲醇处理,使蚕丝素蛋白膜的构象由random coil向β-sheet发生根本性的变化,从而将酶固定在β-sheet所特有的分子间氢键中.利用此方法所制成的脲酶电极,在合适的操作条件下,各项响应指标均令人满意,并且脲酶的耐温性能被大大提高,电极的有效使用寿命长达三个月以上.此种酶固定化方法原则上能够应用于其它不破坏蚕丝素蛋白分子结构的可溶性酶.
A novel method for enzyme immobilization was developed, which is based on the structural transition of the silk fibroin membrane from random coil or α-helix to β-sheet by using methanol, the enzyme was immobilized in the spaces formed by the intermolecular hydrogen bonds in the β-sheet conformation. The resulting urease electrode performs satisfactorily under appropriate operation conditions. It exhibits excellent thermal stability and over three months useful lifetime. In principle, this method should be applicable to immobilize other soluble enzymes which do not destroy the molecular structure of silk fibroin.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
1995年第9期883-888,共6页
Acta Chimica Sinica
基金
国家自然科学基金
上海市自然科学基金资助的项目
