摘要
本文从构建ｃＤＮＡ有限文库中分离出含有全部编码区＋４／＋２８３０ｂｐ的Ｈｓｐ９０αｃＤＮＡ，经ＤＮａｓｅⅠ有限酶解片段的定向亚克隆和序列测定，获得了含有人Ｈｓｐ９０α的ｃＤＮＡ全序列克隆。
recombinant human heat shock
protein(Hsp ) 90α cDNA covering the complete coding se-quences and a part of the5’and 3’
untranslated region(+4/+2830bp ) was efficiently screenedfrom a limited cDNA library and
sequenced through subcloning directionally forced by DNase Ⅰdigestion in the presence of Mn￣
(2+).
出处
《基础医学与临床》
CSCD
1995年第2期103-107,共5页
Basic and Clinical Medicine
基金
卫生部项目基金
自然科学基金(新概念
新构思)
关键词
热休克蛋白
定向亚克隆
脱氧核糖核酸
Heat shock protein 90α cDNA DNase Ⅰ diretional subcloning
