摘要
目的研究莪术中姜黄素在大鼠体内的药动学.方法采用高效液相色谱法测定姜黄素的血药浓度.色谱柱:Lichrospher-5-C18(250 mm×4.6 mm,5 μm);柱温:25℃;流动相:乙腈-5%醋酸水(45:55);体积流量:1 mL/min;检测波长:420 nm.结果姜黄素在6.5～104 μg/mL与峰面积呈良好的线性关系(r=0.999 5).平均回收率为98.5%,RSD为2.41%(n=5).采用药动学计算程序处理,姜黄素的药动学参数:ka为0.53/h、ke为0.10/h、t1/2ka为1.32 h、t1/2k为6.89 h、tpeak为3.89 h、Cmax为93.15 ng/mL、AUC为1 369.38 ng/mL.结论本法稳定、简单、可靠,可用于姜黄素的血药浓度分析及其药动学研究.
Objective To study pharmacokinetics of curcumin in Curcuma phaeocaulis in rats in vivo. Methods HPLC method was used to determine the curcumin in rat plasma. The conditions were column; Lichrospher-5-C18 （250 mm × 4.6 mm, 5 μm） ; column temperature： 25 C ; mobile phase： CH3CN-5% HAc water solution （45 ： 55）; flow： 1 mL/min; detection wavelength： 420 nm. Results The calibration curve was liner （r=0. 999 5） at the range of 6.5-104 μg/mL. The average recovery was 98.5%. RSD was 2.41% （n=5）. The pharmacokinetic parameters of curcumin were as follows： ka was 0.53/h, ke was 0. 10/h, t1/2ka was 1. 32 h, t1/2k was 6. 89 h, tpeak was 3. 89 h, Cmax was 93.15 ng/mL, AUC was 1 369.38 ng/mL. Conclusion This method is stable, simple, and reliable, which can be applied for the determination of curcumin in plasma and oharmacokinetic studies.
出处
《中草药》
CAS
CSCD
北大核心
2005年第8期1157-1158,共2页
Chinese Traditional and Herbal Drugs
基金
国家"十五"攻关课题(2001BA701A11)
