摘要
目的了解D6S477等5个基因座在青岛地区汉族群体中基因型分布及等位基因频率等遗传多态性数据,初步探讨其应用价值。方法收集200名青岛地区汉族无关个体的静脉血,ACD抗凝,采用Chelex法提取DNA,应用聚合酶链反应技术,扩增D6S477、D9S1118、D18S865、D19S400和D20S161基因座的短串联重复序列,聚丙烯酰胺凝胶垂直电泳,银染显色分型。结果获得了青岛地区汉族群体上述5个基因座的等位基因频率,基因型分布均符合HardyWeinberg平衡(P>0.05)。结论这5个基因座在青岛地区汉族群体中有较高的非父排除率和个人识别机率,在遗传学研究中有较高的应用价值。
Objective To illuminate the preliminary genotype and allele frequency distribution of D6S477 and the other four short tandem repeat（SIR） loci in Chinese Han population in Qingdao area and to probe the possibility of their genetic application. Methods Two hundred ACD-blood specimens were collected from the tmrelated individuals in Qingdao . The DNA samples were extracted with Chelex method and were amplified by polymerase chain reaction technique. The PCR products were analyzed by polyacrylamide gel electrophoresis and displayed using silver staining. Results The authors obtained the allele frequency distribution and preliminary genotype of D6S477, Dgs1118, D18S865,D19S400 and D20S161 STR loci. No deviation from Hardy-Weinberg equilibrium was observed in the five loci. Conclusion All the five loci have higher chance of exclusion and discriminating power, and they will be useful markers for researches in genetics.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2005年第4期473-475,共3页
Chinese Journal of Medical Genetics
基金
青岛市科技局项目基金(022KJyn231
2)~~
