摘要
目的观察系统性红斑狼疮(SLE)患儿单个核细胞(MNCS)凋亡的变化,并检测周围血中细胞因子IL-8、IL-6、TNF-α、NO和黏附分子P-选择素(P-sel)、细胞间黏附分子-1(ICAM-1)水平的变化,探讨细胞因子和黏附分子对MNCS凋亡的影响。方法采用流式细胞术检测32例SLE病人和10例健康对照者MNCS的凋亡情况,ELISA法检测细胞因子和黏附分子水平。结果活动期SLE患者MNCS凋亡率明显高于健康对照组和缓解期患者,缓解期患者MNCS凋亡率与对照组无差别,活动期患者周围血中IL-8、IL-6、TNF-α、NO、P-sel、ICAM-1水平均高于对照组和缓解组,且与MNCS凋亡和病情均呈正相关。缓解组患者IL-8、IL-6、TNF-α、NO、P-sel和ICAM-1与对照组无显著性差异。结论SLE患者MNCS凋亡增加,且与病情及疗效密切相关。炎性细胞因子产生过多、免疫细胞黏附分子表达上调可能是导致MNCS凋亡增高的重要机制,通过适度调控细胞因子的分泌和MNCS凋亡有可能会改善SLE预后。
Objective To explore relationship between the changes of mononuclear cells （MNCS） apoptosis and the levels of inflammatorycy tokines and adhesion molecules in patients with systemic lupus erythematosns（SLE）. Methods The apoptotic rates of MNCS in 32 patients with SLE and 10 healthy subjects were detected with flow Cytometry, and the plasma levels of inflammatory cytokines（IL- 8, IL- 6,TNF- a and NO）and adhesion molecules（ P - Sel, ICAM - 1 ） were assayed with ELISA. Results The percentage of MNCS apoptosis in the patients with active stage of SLE was obviously higher than that in healthy subjects and patients with remission stage of SLE, and there was no significant difference in the percentage of MNCS apoptosis between healthy subjects and patients with remission stage of SLE. The levels of plasma IL - 8, TNF - α, IL - 6, NO, P- sel and ICAM - 1 in patients with active stage of SLE were significantly higher than those in healthy subjects and the patients with remission stage of SLE, and were positively correlated with the percentage of MNCS apoptosis and the severity of SLE. There was no significant difference in the levels of plasma inflammatory cytokines and adhesion molecules between patients with remission stage of SLE and healthy subjects. Conclusion The apoptotic rate of MNCS increased in the patients with active SLE, and was closely associated with the severity and efficacy of SLE. The high expressions of inflammatory cytokines and adhesion molecules may be the reason of MNCS apoptotic increase. Appropriately regulating the expression of cytokines and adhesion molecules, and moderately controlling the apoptosis of MNCS may improve the prognosis of SLE.
出处
《中国医师杂志》
CAS
2005年第8期1011-1013,共3页
Journal of Chinese Physician
