期刊文献+

脂肪颗粒组织在不同冻存条件下低温保护剂的筛选 被引量:7

Selection of cryoprotector in preservation of fat granule at different frozen conditions
下载PDF
导出
摘要 目的:寻求不同温度下脂肪组织冻存时的适宜低温保护剂。方法:在-16℃(普通家用冻箱)、-80℃(深低温冰箱)及-196℃(液氮)条件下,加入不同的低温保护剂,冷冻脂肪组织,24h后测定肌酸激酶活力,并与贮存前酶活力做比较。结果:液氮组以15%二甲基亚砜,6%丙二醇为低温保护剂时脂肪组织活力最高,达贮存前的76%,深低温冰箱组以15%二甲基亚砜组活力最高,达66%,而普通冰箱组最高也仅为46%。结论:低温保护剂能明显保持冷冻脂肪组织活力,不同冻存条件下其最适宜的低温保护剂各异。 Objective To explore an appropriate cryoprotector for cryopreservation of adipose tissue. Methods Treated with different cryoprotectors,fat granule was stored at -16℃(domestic refrigerator),-80℃(ultra-low-temperature refrigerator)and -196℃(liquid nitrogen)respectively.Activity of creative kinase was measured 24 hours later,and contrasted with that of fresh adipose tissue. Results At -196℃,while cryoprotectors was 15% dimethyl sulphoxide and 6% propanediol,the viability of adipose tissue was the best,which reached to 76%.At -80℃,the highest viability of adipose tissue was 66% with the use of 15% dimethyl sulphoxide.However,at -16℃,the best result was only 46%. Conclusion The appropriate cryoprotector could obviously keep the viability of frozen fat,it should be chosen according to the different frozen condition.
出处 《中国美容医学》 CAS 2005年第4期397-399,共3页 Chinese Journal of Aesthetic Medicine
关键词 脂肪颗粒 冻存 低温保护剂 fat granule cryopreservation cryoprotective agents
  • 相关文献

参考文献7

二级参考文献43

  • 1刘丽均,徐平.小鼠胚胎玻璃化冷冻的研究进展[J].中国实验动物学杂志,2001,11(2):115-117. 被引量:23
  • 2鄂征.组织培养技术[M].北京:人民卫生出版社,1993.185-189.
  • 3Chajchir A, Benzaqueen I. Liposuction fat grafts in face wrinkle and hemifacial atrophy [J]. Aesthetic Plast Surg, 1986,10(5):55-59.
  • 4Billings Ejr, May Wjr. Historical review and present status of free fat graft autotransplantation in plastic and reconstructive surgery [J]. Plast Reconstr Surg, 1989,83:368-378.
  • 5Illouz YG."The fat cell graft"a new technique to fill depressions [J]. Plast Reconstr Surg, 1986,78:118-126.
  • 6朱兆明 盛志勇.液氮储存皮肤及其使用经验[J].中华医学杂志,1982,:62-560,559.
  • 7Foumier PF. Facial recontouring with fat grafting [J]. Dermatol Clin, 1990,8(3):523.
  • 8Sattler G, Sommer B. Liporecycling: immediate and delayed [J]. Am J Cosmet Surg, 1997,14:311.
  • 9Jackson RF. Frozen fat: does it work?[J]. Am J Cosmet Surg,1997,14:339.
  • 10MacRae JW, Tholpady SS, Ogle RC, Morgan RF. Ex vivo fat graft preservation: effects and implications of cryopreservation.[J].Ann Plast Surg, 2004,52(3):281-282, Discussion 283.

共引文献34

同被引文献55

引证文献7

二级引证文献33

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部