摘要
目的研究香烟烟气溶液和亚砷酸钠联合作用对大鼠淋巴细胞活力和氧化应激反应的影响,并探讨两者之间是否存在交互作用。方法香烟烟气溶液和亚砷酸钠单独作用或联合作用于大鼠淋巴细胞,用AlamarBlue还原法定量评价细胞活力变化,用细胞内还原型谷胱甘肽含量和丙二醛含量评价细胞内的氧化应激反应,并利用2×3析因设计研究两者的交互作用。结果染毒各组的AlamarBlue还原率以及细胞内还原型谷胱甘肽含量显著降低,细胞内丙二醛含量显著升高。析因设计分析结果表明,香烟烟气溶液和亚砷酸钠对AlamarBlue还原率的影响不存在交互作用,而对细胞内还原型谷胱甘肽含量和细胞内丙二醛含量的影响存在交互作用。结论在本研究中香烟烟气溶液和亚砷酸钠单独作用或联合作用均能够降低大鼠淋巴细胞活力,增强细胞内的氧化应激反应,且两者对细胞内的氧化应激反应的影响存在交互作用。
Objective To investigate the effect of cigarette smoke solution and sodium arsenite on viability and oxidative stress of rat lymphocytes and to study their interaction. Methods Cigarette smoke solution with or without sodium arsenite were added to rat lymphocytes. Alamar Blue reduction rate was detected and used to evaluate viability of cells; Reduced glutathione and malondialdehyde were detected and used to evaluate oxidative stress; 2×3 factorial design was used to evaluate the interaction. Results Alamar Blue reduction rate and reduced glutathione of all experimental groups were significantly lower, whereas malondialdehyde was significantly higher. The results of the factorial design showed：Cigarette smoke solution and sodium arsenite had no interaction on Alamar Blue reduction rate, while, they had the interaction on reduced glutathione and malondialdehyde. Conclusion Cigarette smoke solution with or without sodium arsenite decrease viability of cells and raise their oxidative stress, moreover, they have the interaction on oxidative stress of cells.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2005年第8期915-916,共2页
Chinese Journal of Public Health
关键词
香烟烟气溶液
亚砷酸钠
细胞活力
氧化应激
交互作用
cigarette smoke solution
sodium arsenite
cell viability
oxidative stress
interaction
