摘要
目的:研究PTEN抑癌基因在乳腺癌组织中的表达,探讨其与乳腺癌病人的外周血、骨髓及前哨淋巴结微小转移灶之间的关系。方法:选择53例乳腺癌病人的组织标本,用免疫组织化学方法检测原发肿瘤PTEN蛋白的表达;用定量RT鄄PCR法测定原发肿瘤PTENmRNA的表达。以免疫细胞化学法检测外周血和骨髓中的微小转移灶;HE染色和免疫组织化学法检测前哨淋巴结中的微小转移灶。结果:外周血、骨髓及前哨淋巴结中微小转移灶的检出率分别是24.5%,56.6%,26.4%和41.5%。乳腺癌组织中PTEN蛋白表达呈丢失者占35.8%,后者与外周血和骨髓微小转移灶间无显著关系,而与前哨淋巴结中的微小转移密切相关(P≤0.001)。PTENmRNA的表达与外周血、骨髓及前哨淋巴结中的微小转移灶之间均无显著相关性。结论:乳腺癌组织中PTEN蛋白表达的丢失与前哨淋巴结中的微小转移有密切关系,可作为预测其早期转移的重要指标。
Objective To assess the ability of expression of PTEN as a predictor for micro-metastasis in breast cancer patients. Methods As destination sites for micro-metastasis, peripheral blood （BD）, bone marrow （BM） and sentinel lymph node （SLN） from 53 breast cancer patients were examined. Protein and gene expressions of PTEN at the primary site were determined by immunohistochemistry （IHC） and real-time reverse transcript polymerase chain reaction（RT-PCR） respetivdy. BD and BM samples were processed using immunocytochemistry （ICC）. SLNs were examined by hematoxylin and eosin （HE） staining and IHC. Results The percentage of micro-metastasis was 24.5% for BD, 56.6% for BM, 26.4% in SLN by HE and 41.5% in SLN by IHC. Loss of PTEN expression was significantly associated with SLN micro-metastasis as shown both by HE and IHC （P〈O.O01）. On the contrary, there was no significant correlation between PTEN expression and micro-metastasis in the BD and the BM. Meanwhile, there was no significant correlation between PTEN gene amplification and the presence of micro-metastasis in different homing sites. Conclusions Loss of PTEN expression in the primary tumor is significantly correlated with SLN micro-metastasis but not with BD and BM micrometastasis from patients with breast cancer. Loss of PTEN might predict the risk for SLN micro-metastasis in breast cancer patients.
出处
《外科理论与实践》
2005年第4期360-363,共4页
Journal of Surgery Concepts & Practice
