摘要
目的探讨血管内皮细胞生长因子(vascularendothelialcellgrowthfactor,VEGF)在异体皮质骨移植修复骨缺损中的作用。方法新西兰大白兔45只,雌雄不限,体重2.5～3.0kg。制成双侧桡骨中段1.5cm骨缺损模型,随机均分为3组:对照组单用异体皮质骨移植;实验组用异体皮质骨移植+重组人VEGF局部注射;拮抗组用异体皮质骨移植+VEGF多克隆抗体腹腔注射。于术后1、3、5、8和16周行X线片、病理切片、血管密度免疫组织化学染色和四环素荧光成骨检测,评估骨缺损的修复情况。结果血管密度免疫组织化学染色显示实验组有大量血管生成,且随着时间延长血管数量逐渐增加,于第8周达高峰;四环素荧光标记显示结果与血管密度免疫组织化学染色相吻合。周围软组织血管密度计数及骨形成速率,除第8、16周实验组与对照组差异无统计学意义(P>0.05)外,其余各时间点实验组均优于对照组和拮抗组,且差异有统计学意义(P<0.05);对照组各指标优于拮抗组,差异有统计学意义(P<0.05)。结论VEGF能加速移植的异体骨血管化和爬行替代,促进骨缺损修复。
Objective To study the effect of vascular endothelial cell growth factor （VEGF） on repair of bone defect with cortical bone allograft. Methods Forty five New Zealand white rabbits, weighted 2.5-3.0 kg, were made bone defect model of 1.5 cm in length in the bilateral radii and then were randomly divided into 3 groups. The defect was repaired with only cortical bone allograft in the control group, with the cortical bone allograft and local injection of human recombinant VEGF in the experimental group, and with the cortical bone allograft and abdominal injection of VEGF PAb3 in the antagonist group. Roentgenography, immunohistochemical staining and tetracycline labelling were carried out to evaluate the reparative results 1, 3, 5, 8 and 16 weeks after operation. Results Immunohistochemical staining results showed that a great deal of blood vessels formed in the experimental group, and the number of blood vessels increased gradually with the time and reached the highest value at the 8th week. Tetracycline labelling showed the same result. The best results in callus formation, ossification rate and count of microvascular density were shown in the experimental group, while those in the control group were significantly better than those in the antagonist group （P〈0.0,5）, but there was no significant difference between the experimental group and the control group at the 8th week and the 16th wcek （P〉0.05）. Conclusion VEGF can accelerates the bone formation and angiogenesis in the bone allografts, thus it can promote the repair of bone defects.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2005年第8期593-596,共4页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
血管内皮细胞生长因子骨缺损异体皮质骨移植
Vascular endothelial cell growth factor Bone defect Cortical bone allograft
