摘要
目的研究3D-SC人工皮肤材料在体外的降解性能。方法将3D-SC人工皮肤材料分别置于生理盐水(对照组)、胰组织液(实验1组)、生理缓冲液(Hanks液,实验2组)和pH7.4的0.2mol/L磷酸盐缓冲液(实验3组)中,37℃恒温水浴条件下降解,实验1组第3、5、7、9、11和14天取材,其余各组第3、7、14、15、21和30天取材,测定质量损失百分率。结果材料在实验1组降解最快,14d内完全降解;对照组、实验2组和实验3组,第14天质量损失百分率分别为8.68%±2.30%、28.51%±10.68%和7.35%±0.61%,第15天分别为71.83%±2.58%、91.32%±1.87%和75.64%±6.13%,呈急速式降解,实验2组与对照组比较差异有统计学意义(P<0.01);第30天质量损失百分率分别为91.87%±8.15%、95.62%±1.36%和92.10%±2.26%,3组差异均无统计学意义(P>0.05)。结论3D-SC人工皮肤材料具有较好的降解性。在无酶参与时,降解速度呈慢-快-慢的过程。
Objective To study the degradable properties of 3D-SC artificial skin in vitro. Methods The 3D-SC artificial skin materials were respectively immersed into the solutions of 0. 9% normal saline （control group）, pancreatic tissue liquid （experimental group 1）, physiological buffer （Hanks balanced salt solution,experimental group 2） and 0.2 mol/L phosphate buffer （pH 7.4,experimental group 3）, and the degradation was carried out at 37±℃. The quality lost ratios were dctermined on the 3rd day, the 5th day, the 7th day, the 9th day, llth day and 14th day in the experimental group 1, while on the 3rd day, 7th day, 14th day, 15th day, 21st day and 30th day in the other groups. Results The 3D-SC artificial skin was degraded completely in pancreatic tissue liquid about within 14 days in the experimental group 1; in the control group, and in the experimental groups 2 and 3, the degradation ratios were 8.68%±2.30%,28.51±10.68% and 7.35%±0.61% on the 14th day; 71.83%±2.58%, 91.32%±1.87% and 75.64%±6. 13% on the 15th day, being significant difference between the control group and the experimental group 2 （P〈0.01）; and 91.87%±8.15%, 95.62^±1.36% and 92.10%±2.26% on the 30th day, being no significant differences between these 3 groups（P〉0.05）, respectivelies. Conclusion The 3D-SC artificial skin materials have good degradable properties. The trend of degradation speed is from slow to quick and then to slow without enzyme.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2005年第8期658-661,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家高技术研究发展计划(863)资助项目(2001AA326090)~~
关键词
人工皮肤体外降解胰组织液
Artificial skin Degradation in vitro Pancreatic tissue liquid
