摘要
通过UVB照射培养和热冲击处理,成功地选育到一株36℃下在含16%(v/v)酒精的培养基中生长良好,三角瓶发酵酒精度达到14.3%(v/v)的耐酒精和耐温的酵母菌株UV-7-36-12-17菌。其最佳发酵条件:原料加水比1∶2.2;糖化酶用量125u/g原料,60℃糖化30～60min;接种量8%～10%;摇床转速70～96r/min;发酵顶温34～36℃,发酵周期72h。
An ethanol-resistant and heat-resistant mutant （UV-7-36-12-17） ofSaccharomyces cerevisiea was successfully bred by the techniques of UVB radiation culture and heat-shock treatment, which could grow well in the triangular flask under the conditions of 16 % （v/v）ethanol content, temperature at 36℃, and alcohol content reached 14.3 % （v/v）. The optimal fermentation conditions of UV-7-36-12-17 were as follows ： the ratio of raw material and water as 1：2.2; use level of saccharifying enzyme as 125 u/g raw materials; 30～60min saccharification at 60℃ ; 8 %～10 % inoculation quantity; shaker rotate speed as 70～96 r/min; the top temperature in fermentation at 34～36℃ ; and fermentation cycle as 72 h.（Tran. by YUE Yang）
出处
《酿酒科技》
北大核心
2005年第8期35-38,41,共5页
Liquor-Making Science & Technology
关键词
微生物
UVB照射培养
热冲击处理
浓醪
酒精发酵
microbe
UVB radiation culture
heat-shock treatment
high gravity mash
ethanol fermentation
