摘要
伊尼奥小单孢菌F003的发酵特性研究表明,西索米星发酵过程具有典型的非生长耦联特征.当以淀粉为主要碳源时,对数生长期最大菌体比生长速率为0.058 h-1,产物合成期最大产物比合成速率0.0018 g/(g·h).HPLC和酶法测定结果显示发酵液中主要的可发酵糖为麦芽糖,适合菌体生长的麦芽糖浓度或葡萄糖浓度分别为10.0～25.0和7.5～15.0 g/L,可发酵糖浓度低于10.2 g/L将限制西索米星合成.西索米星发酵过程淀粉水解酶表观活性的动力学特性表明,在对数生长期淀粉水解酶表观活性较高,最大值为0.84 g/(L·h),在发酵中后期淀粉水解酶表观活性仅为最大值的1/4～1/20,导致可发酵糖浓度降低,限制西索米星的合成.西索米星浓度大于0.50 g/L将明显抑制产物合成.
The sisomicin production was found to be non-associated with the cell growth of M. inyoensis F003. When starch was used as main carbon source, the maximal specific growth rate was 0.058 h^-1, and the maximal specific sisomicin production rate was 0.0018g/（g·h）. The cells of M. inyoensis F003 grew well at 10.0～25.0 g/L maltose or 7.5～15.0 g/L glucose in fermentation broth. It was shown by HPLC analysis and enzymatic measurement that maltose was the main fermentable sugar during the fermentation, and sisomicin biosynthesis was limited when the level of fermentable sugar was below 10.2 g/L. The apparent activity of starch-hydrolytic enzymes in the batch fermentation of M. inyoensis F003 was 1/4 to 1/20 fold of the maximum value 0.84 g/（L·h） in sisomicin biosynthesis phase, its apparent activity, which would result in restriction to the sisomicin biosynthesis by the limitation of fermentable sugar in the broth. Sisomicin above 0.5 g/L was inhibitive to its biosynthesis.
出处
《过程工程学报》
EI
CAS
CSCD
北大核心
2005年第4期430-433,共4页
The Chinese Journal of Process Engineering
基金
福建省自然科学基金资助项目(编号:C0210007
B9810007)
福建省教育厅科技基金资助项目(编号:K20011)
