摘要
目的观察脂质体瘤苗的抗癌作用,寻找有效的抗肿瘤疫苗。方法制备H22肝癌细胞的脂质体瘤苗,将该瘤苗通过腹腔接种,免疫荷瘤的BalB/c小鼠(LAg组),同时设单用抗原免疫组(Ag组)和DHanks组作为对照,比较各组小鼠的生存期,并用MTT法检测外周血和脾淋巴细胞对H22肝癌细胞的体外杀伤作用。结果LAg免疫的BalB/c鼠的平均生存期及脾和外周血淋巴细胞毒活性均高于Ag组及DHanks组(P<0.05),Ag组及DHanks组两者之间差异无显著性(P>0.05)。结论单用H22抗原不能诱导有效的抗肝癌免疫反应,用脂质体包裹后,其抗肝癌作用可明显增强,脂质体瘤苗有潜在的临床应用前景。
Objective To observe the antitumor effect of liposomal tumor vaccine and explore the effectice tumor vaccine formulation. Methods The soluble antigen of H22 hepatoma （Ags） was extracted using 3M KC1 and purified by centrifugation and dialysis. This antigen was entrapped into the MLVs （muhilamellar vesicles） liposomes by mechanical dispersion to constitute the liposomal tumor vaccine. Sixty BalB/c mice inoculated with H22 Hepatoma cell beforehand were divided into three groups at random and were inoculated intraperitoneally with liposomal tumor vaccine （LAg group）, the soluble antigen of H22 hepatoma（Ag group）and D-Hanks（D-Hanks group）, respectively, eight days later reinoculated. Their survival time was observed and the lymphocyte cytotoxicity was valued by MTF assay. Results LAg group had a longer survival periods and stronger lymphocyte cytotoxicity compared with those treated with Ag and D-Hanks （ P 〈 0.05）, but the difference between Ag group and D-Hanks group were not significant （ P 〉 0.05）. Conclusion The antitumor immune response of mice with tumor elicited by H22 Ags entrapped in liposomes was enhanced significantly, although no effective response was induced by H22 Ags only,
出处
《胃肠病学和肝病学杂志》
CAS
2005年第4期366-368,共3页
Chinese Journal of Gastroenterology and Hepatology
