摘要
利用溶胶-凝胶技术制得了单分散性很好的二氧化硅纳米微球,通过共价偶联方式引入对pH变化敏感的荧光素异硫氰酸酯(FITC),这种FITC功能化二氧化硅纳米微球(FITC功能化硅微球)可作为pH荧光纳米传感器。当pH值从4.0变至7.5时,荧光强度增大约30倍。该传感器灵敏度高,响应速度快,容易制备,光稳定性好,可望用于单细胞的pH分析。
Mono-dispersed silicon dioxide spherical nanoparticles have been prepared by sol-gel method and a novel fluorescence pH nanosensor was developed with surface modification of fluorescein isothiocyanate（FITC）. The nanosensor is sensitive over the pH range 4.0 - 7.5. When the pH value changes from 4.0 to 7.5, the fluorescence intensity of this nanosensor increases about 30 times. As a pH sensor, it has several unique advantages in high sensitivity, rapid response times, easy preparation and good photostability. It can also be further used as pH probe in single-cell analysis.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2005年第8期1119-1121,共3页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.20275028)
"863"计划(No.2002AA2Z2004)资助项目
