摘要
目的:研究氟对大鼠肝脏氧化应激及超微结构的影响。方法:在饮水中加入不同剂量的氟化钠(0,50,100和150mg/L)喂饲大鼠3个月后,测定肝脏丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,并用透射电镜观察肝脏超微结构的改变。结果:与对照组比较,染氟组大鼠肝脏MDA含量升高,SOD、GSH-Px活性下降。透射电镜下肝细胞染色质边集于核膜下,线粒体肿胀,嵴断裂或消失,内质网扩张,胞质内多见脂滴。结论:过量氟使大鼠肝脏处于氧化应激状态,损伤细胞膜性结构和DNA,造成肝脏损伤。
Objective： To study the effect of fluoride on oxidative stress and ultrastructure in the liver of rats。 Methods： Rats were given sodium fluoride of various concentration in drinking water for 3 months. The contents of malonyl dialdehyde （MDA） and activities of antioxidases were measured and the ultrastruture changes were observed with TEM， Resuits： Compared with the control group, the content of MDA increased and the activities of SOD and GSH-Px decreased in fluoride treated groups. The uhrastruetural changes included condensation and margination of ehromatin, swollen mitoehondria, and enlargement of endoplasmie retieulum. Conclusion： Excessive fluoride may induce oxidative stress and lesions ofmembrane and DNA in the liver of rats。
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2005年第4期321-322,共2页
Journal of China Medical University
关键词
氟中毒
肝脏
氧化应激
超微结构
fluorosis
liver
oxidative stress
ultrastructure
