摘要
本文应用吸虫器法与剥查法,研究了转Cry1Ac+SCK基因水稻及其杂交后代对水稻害虫群落的影响。结果显示:转Cry1Ac+SCK基因水稻MSA与MSB对稻纵卷叶螟具有极强的抗性,转Cry1Ac+SCK基因稻的杂交后代21S/MSB与Ⅱ-32A/MSB都获得了对稻纵卷叶螟的高抗性。虽然MSA、MSB、21S/MSB与Ⅱ-32A/MSB对水稻害虫的物种丰富度、个体数量、多样性与均匀度时间动态都有一定程度的影响,但生长全期的调查结果显示,与对应的对照比较,它们对水稻害虫群落的这些参数均无显著影响,且转基因水稻田与各自对照田在害虫与主要害虫物种组成上具有较高的相似性。靶标害虫种群数量的降低,并未对水稻害虫群落的组成与结构产生明显的影响,也没有引起优势种成分的变化。
Effect of MSA and MSB, transgenic rice with fused genes of CrylAc and SCK, and 21S/MSB and Ⅱ-32A/MSB, progenies from crosses between Cry1 Ac/SCK-transgenic rice and conventional rice varieties on the pest insect community in paddy field was tested by the suction machine sampling method combined with the field denudation method. The results indicated that both MSA and MSB were highly resistant against the rice leaf roller, Cnaphalocrocis medinalis. The rice lines 21S/MSB and Ⅱ-32A/MSB also obtained the trait of high resistance to C. medinalis. Although MSA,MSB, 21S/MSB and Ⅱ-32A/MSB showed some effect on the temporal dynamics of species richness, number of individuals, diversity, evenness and dominant concentration indices, the results from analysis of the whole period of ricegrowing revealed that the four rice varieties had no remarkable effect on the species richness, total number of individuals,diversity, evenness and dominant concentration indices of communities of pest insects in paddy fields compared with their respective control. Comparison of the results of different rice revealed high compositional similarities in the total and key pest insect species. The removal or depressing of population of target pest insect C.medinalis had negligible effect on the dominant species, composition, and structure of pest insect communities in paddy fields planted with CrylAc/SCKtransgenic rice.
出处
《昆虫学报》
CAS
CSCD
北大核心
2005年第4期544-553,共10页
Acta Entomologica Sinica
基金
国家自然科学基金项目(30270879)
国家"863"项目(2002AA22031)
湖南省杰出青年科学基金项目(04JJ1003)
福建省科技攻关计划项目(2003N002)
福建省科技重大专项(2004NZ01-2)
