摘要
目的探讨基质金属蛋白酶2、9(MMP-2、9),膜型基质金属蛋白酶1(MT1-MMP)及基质金属蛋白酶组织抑制剂1、2(TIMP-1、2)与涎腺恶性肿瘤浸润生长的关系。方法应用免疫组化SP法和明胶酶谱法检测28例涎腺良性肿瘤、26例涎腺恶性肿瘤中MMP-2、9,MT1-MMP及TIMP-1、2的表达及细胞定位,分析其中酶原与活性酶的含量比例。结果涎腺恶性肿瘤中MMP-2、9的表达强于良性肿瘤(P<0·05),TIMP-1、2的表达低于良性肿瘤(P<0·05)。MMP-2、MT1-MMP、TIMP-2的表达有相关性。MMP-9酶原及活性MMP-9在恶性肿瘤中的表达均高于良性肿瘤(P<0·05)。结论MMP-2、9在涎腺恶性肿瘤浸润性生长中扮演了重要角色。涎腺恶性肿瘤中,TIMP-1、2抑制作用的降低导致了MMP-2、9合成、激活的增多,令MMP-2、9与其天然组织抑制剂TIMP-1、2之间的平衡失调,从而基底膜加速降解。
Objective To detect protein expression of MMPs and TIMPs in various salivary gland neoplasms and to investigate their roles in invasion and metastasis of the malignant salivary gland tumors. Methods Immunohistochemistry and Gelatin zymography analyses for MMP-2, MMP-9, MT1-MMP, TIMP-1 and TIMP-2 were performed in 26 malignant and 28 benign salivary gland tumors. Results The expression of MMP-2 and MMP-9 was significantly higher in carcinomas than in adenomas （ P 〈0.05）. The MMP-2/TIMP-1 and MMP-2/TIMP-2 was also significantly higher in carcinomas than in adenomas（ P 〈 0.05）. There was a cooperated effect among MMP-2, MT1-MMP and TIMP-2. The expression of active MMP-2, pmMMP-9 and active MMP-9 was significantly higher in malignant tumors than in benign tumors （ P 〈 0.05 ）. Conclusion MMP-2 and MMP-9 may play impertant roles in invasion of malignant salivary gland tumors. A disturbed balance between MMP-2, MMP-9, TIMP-1 and TIMP-2 in malignant salivary gland tumors was detected. It was the absolute increase of MMP-2 and MMP-9 to induce the unbalance.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2005年第4期273-276,279,共5页
West China Journal of Stomatology
基金
教育部回国人员科研启动基金资助项目(0040305402002)
关键词
基质金属蛋白酶
基质金属蛋白酶组织抑制剂
涎腺肿瘤
matrix metalloproteinases
tissue inhibitors of metalloproteinase
salivary gland tumor
