摘要
从发病鸡群中分离的产蛋下降综合症病毒（ＥＤＳ＿（－７６））Ｈ＿（９１）株接种鹅胚收获的尿囊液，用氯化铯密度梯度离心等方法纯化了ＥＤＳ病毒。电镜下观察到病毒无囊膜，大小为７０～８５ｎｍ。从纯化的病毒悬液提取的核酸，经琼脂糖凝胶电泳只出现一条分子量为３４ｋｂ、背景清晰的核酸带。用６种限制性内切酶对其基因组ＤＮＡ进行了酶切分析，各种酶消化分别产生４，４，９，９，１１和３条片段。将此结果与ＥＤＳ标准株ＡＶ＿（－１２７）株基因组ＤＮＡ由相同的内切酶消化的结果进行比较发现，由ＨｉｎｄⅢ和ＳｍａⅠ消化２个病毒基因组ＤＮＡ产生的片段数及大小有些差异。
By using cesium chloride gradient ultra-centrifugation,egg drop syndrom virus (EDS)virionswere purified from allantoic fluids of goose embryos inoculated with the EDS strian H_(91) isolated from a layerflock. Under electronic microscope, the round virus paticles in diameters of 70～85nm without envelopes wereobserved in the preparation. The phenol-extracted DNA from the purified virus suspension showed a clear sin-gle band in the agarose gel electrophoresis, The viral genomic DNA was cleaved into 4,4,9,9,11 and 3fragments . Molecular weight of viral genomic DNA was found to be 22.42 ×10￣6 dolton respectlvily by diges-tion with restriction enzyme:EcoR Ⅰ,BamH Ⅰ, Hind Ⅲ, Pst Ⅰ, Pvu Ⅱ and Sma Ⅰ. The result was com-pared with digestion of viral genomic DNA of EDS standerd strian AV_(-127) with the same restriction endonu-cleases. It showed that there was some difference in numbers and lengths of fragrnents generated by digestionof viral genomic DNA with Hind Ⅲand Sma Ⅰ between EDS H_(91) strian and EDS AV_(-127) strain.
出处
《江苏农学院学报》
CSCD
1995年第3期54-58,共5页
Jiangsu Agricultural Research
基金
江苏省科委自然科学基金
关键词
鸡病
卵用鸡
减蛋综合症
病毒DNA
酶切分析
domestic fowl
egg+drop+ syndrotn
restriction endonucleases
viral + DNA
