摘要
目的体外研究白细胞介素21(IL-21)对骨髓瘤细胞增殖的促进作用。方法以IL-21及IL-6体外刺激来自于骨髓瘤患者的骨髓瘤细胞,RT-PCR分析IL-21受体(IL-21R)在骨髓瘤细胞中的表达,MTT分析IL-21及IL-6促进骨髓瘤细胞的增殖效应。结果RT-PCR分析表明骨髓瘤细胞表达IL-21R;MTT分析结果显示IL-21体外对11例患者中的7例骨髓瘤细胞具有促进作用,最大增殖率达154%,但其作用强度低于IL-6。结论IL-21具有促进骨髓瘤细胞增殖作用,其作用强度较IL-6弱。
Aim To study the role of Interleukin-21 increased the proliferation of myeloma cells in vitro. Methods The IL-21R transcript was detected in all examined myeloma cells by RT-PCR; IL-21 or/and IL-6 increased the proliferalion of myeloma cells ,as determined by MTT. Results The IL-21R was detected from seven of 11 samples. Seven of 11 samples were responsive to IL-21 （proliferation ratio： 187±26% ）. Nine samples that included those responding to IL-21 ware also responsive to IL-6 （proliferation ratio;219±37% ）. The response magnitude to IL-21 were lower than the one to IL-6. Conclusion The IL-21 can increase the proliferation of myeloma cells in vitro, but the response magnitude were lower than IL-6.
出处
《安徽医药》
CAS
2005年第8期615-616,共2页
Anhui Medical and Pharmaceutical Journal
