摘要
目的采用反相高效液相色谱法测定四季三黄软胶囊中黄芩苷的含量。方法色谱柱为spherisorbC18(4.6mm×250mm,5μm);流动相为甲醇-水-磷酸(48∶52∶0.2),流速为1mL·min-1,检测波长为280nm。结果黄芩苷在5.1～51mg·L-1范围内,浓度与峰面积呈良好的线性关系,r=0.9999,平均回收率为99.1%,RSD为0.7%(n=6)。结论方法简便、快速,重现性好,可用于本制剂的质量控制。
Objective To establish a method for determination of baicalin in Siji Sanhuang Soft Capsules by RP-HPLC. Methods RP-HPLC analysis was carried out on spherisorb C18 column and the mixture of methanol-waterphosphoric acid（48：52：0.2）was used as the mobile phase. The flow rate was 1 mL·min^-1. The detection was at 280 nm. Results The linear responses ranged between 5.1～51 mg·L^-1 of baicalin（ r = 0. 999 9）. The average recovery was 99.1% and the was RSD 0.7%. Conclusions The method is simple, rapid and with good reproducibility. It can be used for the quality control of Siji Sanhuanu Soft Capsules.
出处
《西北药学杂志》
CAS
2005年第4期152-153,共2页
Northwest Pharmaceutical Journal
