摘要
目的:探讨黄芪提取物的免疫调节作用。方法:实验设三个浓度的药物组和空白对照组,采用小鼠内脏体重比、迟发型变态反应(DTH)(足跖增厚法)、ConA诱导的小鼠淋巴细胞转化试验(MTT法)、脾脏抗体生成细胞检测(Jerne改良玻片法);半数溶血值(HC50)的测定;碳粒廓清试验;腹腔巨噬细胞吞噬鸡红细胞实验(半体内法);NK细胞活性测定(乳酸脱氢酶LDH测定法)试验。结果:黄芪提取物对小鼠体重、脏器重比无明显影响;对淋巴细胞转化功能和NK细胞活性及细胞吞噬指数与对照组比较也无明显差异;足跖肿胀度与对照组比较均有显著性差异;其抗体生成细胞数中、高剂量组明显增高,而低剂量组则无显著差别;半数溶血值也明显高于对照;中、高剂量组的巨噬细胞吞噬鸡红细胞的吞噬率和吞噬指数与对照组比较均有显著性差异。结论:黄芪提取物具有一定的免疫调节作用。
Objective： To investigate the immunoregulation of Astragalus extraction. Methods： Three concentrations of Astragalus extraction and a control groups have been divided. The Immune function of Astragalus extraction was studied by a serial experiments in vitro and in vivo include spleen and thymus weight ratio, DTH assay, ConA-induced mice lymphocyte transfonnationtest test, antibodies formation in spleens, HC50, monocyte-Macrophage carbon grain elimination test, Macrophage swallow chicken erythroeyte test, NK cells living activities assay. Results：The results showed that Astragalus extraction was not affected the weight of the mice and spleen and thymus weight ratio, but significant increase toe thickenss. It was non-significant to ConA induced mice Lymphocyte transformationtest. The formation of antibodies is increased in mid and high concentrations comparison with the control, while no-significant changes in low dose; HC50 raised in all three concentrations; ratio of monocyte-macrophage carbon grain elimination has non-significant changes to three treatment groups; Macrophage swallow gallinaceous erythrocyte test showed improve in mid and high dose Astragalus extraction; and NK cells living activities displayed non-significant changes comparing to the control. Conclusion ： Astragalus extraction possesses a potential effect of immune regulation.
出处
《军医进修学院学报》
CAS
北大核心
2005年第4期293-295,共3页
Academic Journal of Pla Postgraduate Medical School
基金
解放军总医院科技成果转化基金资助(03CY08)
关键词
黄芪
神经免疫调节
氧化剂
Astragalus membranaceus
neurimmunodulation
oxidants
