摘要
目的探讨大鼠缺血性急性肾功能衰竭模型建立。方法采用切除大鼠右肾并钳闭左肾动脉的方法,分别在1、6、12、24、36h测定肾功能,观察肾组织形态学改变。结果模型建立后血清尿素氮(BUN)和肌酐(Cr)于再灌注后6h开始上升,于24h达到高峰,肾组织24h后出现肾小管细胞坏死脱落,管腔内可见细胞碎片及管型,管腔明显扩张,肾小球无明显改变。结论通过切除大鼠右肾并钳闭左肾动脉的方法可成功建立缺血性急性肾功能衰竭动物模型。
Objective To establish the rat model of ischemic acute renal failure. Methods Right nephrectomy and occlusion of left renal artery method was used in this experiment. Renal function at 1, 6, 12, 24,36 hour after surgery and pathological changes were observe＆ Results Serum BUN and Creatinine level increased from hour 6 and reached peak concentration at hour 24 after surgery. The renal histopathological changes and necrosis at the epithelia of tubular appeared after 24 hours. Conclusion The method with right nephrectomy and occlusion of left renal artery may establish the rat model of ischemic acute renal failure.
出处
《深圳中西医结合杂志》
2005年第4期197-198,219,共3页
Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
基金
深圳市科技计划项目(JH 200505310606A)
