摘要
蝌蚪提取液（Ｔ－８７１）与ＤＭＥＭ培养基按１：５０（ｖ／ｖ）混合制成Ｔ－８７１培养基。作为研究分化模型的小鼠红白血病细胞（ＭＥＬ）培养于Ｔ－８７１培养基中。结果发现Ｔ－８７１诱导ＭＥＬ发生终末分化，即在其胞质中有血红蛋白合成。用流式细胞仪（ＦＡＣＳ－４２０）分析经Ｔ－８７１培养的ＭＥＬ，证明其Ｐ（５３）蛋白含量降低。由于Ｐ（５３）蛋白是细胞由Ｇ１期转变为Ｓ期所必需的，所以本研究认为ＭＥＬ中Ｐ（５３）蛋白含量降低可能导致细胞周期中Ｇ０＋Ｇ１期细胞的积累和Ｇ２＋Ｍ期细胞的减少。
The tadpole extract(T-871) and DMEM medium were mixed in the ratio of 1:50 (v/v) to form T-871 medium.The murine erythroleukemia cells (MEL),which are used as model for study of induction of cell differentiation,were cultured in T-871 medium.We found that the T-871 might induce MEL to initiate into terminal differentiation. The cytoplasrn of differentiated MEL showed hemoglobin synthesis. When these cells were analyzed by flow cytometry,the results indicated that P53 protein content decreased during differentiation. The P53 protein is essential for the transition from G1 phase to S phase. It may suggest that the decrease of MEL P53 protein will result in the cell accumulation of G0 and G1 phases and the decrease of G2 and M phases in the cell cycle.
出处
《解剖学报》
CAS
CSCD
北大核心
1995年第4期403-405,共3页
Acta Anatomica Sinica
关键词
细胞分化
蝌蚪提取液
红白血病
流式细胞术
Cell differentiation
Tadpole extract
Erythroleukemia cell
Flow cytometry
Murine
