摘要
对如何应用单宁酸提高致密核心小泡电子密度进行了研究,结果发现实验组动物应用单宁酸后,其延髓背角胶状质的超微结构出现了如下特征性变化:(1)膜结构清晰度增加,反差鲜明;(2)大、小致密核心小泡均被媒染,其电子密度显著提高,大致密核心小泡于非突触部位胞吐入细胞间隙内的介质被即时媒染、固定.实验还表明,灌流液单宁酸的浓度低于0.25%时,效果不显著;高于0.5%则出现正常结构析解,故先用含0.25%单宁酸的生理盐水溶液经主动脉缓慢滴入后,再快速输入含0.5%单宁酸的醛固定液效果最好.
We used the tannic acid(TA) to increase the electron density of dense cored vesicles. The results showed that the ultrastructure of the substantia gelatinosa in medullary dorsal horn changed characteristically after the application of TA to the experimental animals. (1) The structure of plasmalemma was more clearly visulised. (2) Both large and small dense cored vesicles were well mordantly stained by TA, their electron density increased significantly, and the transmitters were stained and fixated promptly as they were released from large dense cored vesicles into intercellular space by exocytosis at the nonsynaptic sites. The experiment also showed that the effect was not obvious when the concentration of perfusion solution containing TA was below 0.25%, but when it was above 0.5% the normal structures were destinctly resolutive and disperse. We found that the ideal result could be obtained by perfusing the animals first with 0.25% TA normal saline solution slowly and then with 0.5% TA double aldehyde solution quickly through the aorta.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1995年第1期44-48,共5页
Chinese Journal of Anatomy
关键词
大鼠
致密核心小泡
胞吐
组织学
单宁酸
rat: large dense cored vesicle
small dense cored vesicl
exocytosis
