摘要
目的:采用HPLC法测定丹参酮的含量,建立消炎汤合剂的质量控制标准.方法:采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm),甲醇-水-乙腈(70:25:5)为流动相,流速1.3 ml/min,检测波长270 nm.结果:丹参酮Ⅱ-A在5～60μg/ml范围内线性关系良好(r=0.999 2),平均回收率为100.41%,RSD为2.26%.结论:本方法具有简便、快速、灵敏、准确、经济等特点,适用于消炎汤合剂的质量控制.
Object ： To develop an HPLC method to determine the content of tanshinone Ⅱ-A for the quality control of the antiphlogistic mixture. Methods： The chromatographic separation was performed on a 4.6 mm ×250 ram, 5μm Kromasil C18 column. The mobile phase consisted of MeOH-water-acetonitrile （70 ： 25 ： 5，v/v/v）， The flow rate was 1.3 ml/min and the UV detection was set at 270 nm. Results： There was a good linearity over the range of 5-60μg for tanshinone Ⅱ-A （ r =0. 999 2）. The average recovery was 100.41% with the RSD of 2. 26%. Conclusion： The method was simple, rapid, sensitive, accurate and economical. It was suitable for the quality control of the antiphlogistic mixture。
出处
《天津药学》
2005年第4期22-24,共3页
Tianjin Pharmacy
