摘要
目的研究无肝期前后与异丙酚代谢相关的尿苷二磷酸葡萄糖醛酸转移酶ugt1a6在大鼠肾脏中的基因表达变化,初步阐释异丙酚无肝期肝外代谢特点形成的原因。方法15只雄性大鼠随机分为3组(5只/组),A组为对照组,B组阻断肝门30 m in,C组阻断肝门60 m in,截取各组大鼠肾脏组织,采用逆转录聚合酶链式反应(RT-PCR)技术,检测各组ugt1a6 mRNA的表达。结果大鼠肾脏组织ugt1a6的基因表达水平B、C组明显高于A组(P<0.05);B组与C组相比,无显著差异(P>0.05)。结论大鼠肾脏组织ugt1a6基因表达在无肝期比无肝期前增多,这可能是异丙酚无肝期肝外代谢增强的机制之一。
Objective To investigate the gene expression variation of rat kidney uridine diphosphate glucuronatetransferase ugtla6 related to propofol metabolism in anhepatic phase and to primarily explain the reasons of extrahepatic metabolism characteristic of propofol. Methods A total of 15 male SD rats were distributed randomly to 3 groups （ n = 5 in each group） ： control group （ group A） , devascularization of the hepatic portal for 30 min （group B） and 60 min （group C）. Kidney tissues of rats were taken and ugtla6 mRNA were detected by RT-PCR. Results Gene expression levels of kidney ugtla6 of groups B and C were significantly higher than group A （ P 〈 0. 05 ）. The gene expression levels between B and C were of no significant difference. Conclusion Gene expression levels of kidney ugtl a6 of rats increased in anhepatic phase as compared with the phase of hepatic portal dissociation, which is one of the possible reasons that extrahepatic metabolism of propofol is increased in anhepatic phase.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2005年第16期1697-1699,共3页
Journal of Third Military Medical University
基金
第三军医大学中青年科研基金资助项目(2004)~~
