摘要
芹菜属于冬性长日植物,它需要经过低温春化阶段才能开花结实,但低温仅是外界条件,还必需通过植物体内部的生理生化变化才能起作用。关于春化作用机理的研究,自 Melchers(1939)提出低温诱导植物形成春化素(vernalin)的假说以后,Purvis等(1953),Highkin(1955)和Tomita(1959,1964)曾自不同植物中分离出能代替低温或促进开花的物质。
A substance identified to be 'zearalenone'was found in overwintering shoot apices of celeryseedlings. It was isolated by TLC and observedunder UV light (254 nm). It has the same R_fvalue with blue fluorescence ps that of the stan-dard zearalenone (a secondary metabolite ofGibberella zeae, mol wt.= 318). HPLC analysisshowed that it has the same retention time (3.01min.) as that of the standard. Their UV spectraare also identical with 3 peaks at 236,274 and316 nm respectively, MS/MS analysis of the cru-de axtract showed that there is a quasimolecu-lar ion of M / Z 319= MH^+, that means the mo-lecular weight of this substance is 318, and itsdaughter spectrum is also identical with that ofthe standard zearalenone., All these indicatethat zearalenone actually occurs in the overwin-tering shoot apices of celery seedlings. It wasalso noticed that zearalenone (and perhaps itsderivatives) which is closely related to vernaliza-tion may occur widely in winter plants. It mayact as a hormonal signal promoting plants to passvernalization period as well as certain necessarystep before flowering. The physiological effectsof zearalenone should be studied further.
基金
国家自然科学基金
关键词
芹菜
春化作用
玉米赤霉烯酮
vernalization
zearalenone
Apium graveoleus L
