摘要
叔丁醇冻结干燥法用于植物材料,获得了良好的效果。经常规固定的样品完成脱水以后浸泡在叔丁醇中,然后将含醇样品放入电冰箱中冷冻,待叔丁醇凝固后再转移到真空镀膜机的钟罩里,用机械泵抽真空,冻结的醇在低真空中升华后样品得到了干燥。SEM 镜检表明,样品的表面和细胞断裂面及其深层(可见到内质网的双层膜、线粒体的嵴、高尔基体潴泡叠层、核糖体颗粒等细胞器)的三维图象均优于用临界点干燥法所得到的样品图象,叔丁醇冻结干燥法用于植物 SEM 样品制备,是一种操作简单,干燥质量可靠、值得推广的方法。
The t-butyl alcohol freeze-drying method (Inoue and Osatake 1988) is a simple drying method of biological materials for scanning electron microscopy.Fixed specimens were im- mersed in t-butyl alcohol after dehydration throgh a graded series of ethanol.Specimens in the alcohol were then forzen in a refrigerator.They were placed in the bell jar of a vacuum evaporator and simply evacuated with a rotary pump.The samples were completely dried within 40—60 min.after the frozen alcohol was sublimated in the vacuum,when the speci- men was examined by scanning electron microscopy (SEM),both surface and intracellular structures were demonstrated in three-dimension without any significant drying artifacts.Ca- reful comparison of the results indicated that the SEM imayes obtained by this method were either superior or equal to those obtained by the critical point drying method.
关键词
叔丁醇
扫描电镜
冻结干燥法
Tertiary butyl alcohol
Scanning electron microscopy (SEM)
Freeze-drying method (FD)
Critical point drying method (CPD)
