摘要
以马尾松种胚作外植体进行不定芽诱导。基本培养基的种类对外植体不定芽的诱导起着决定性的作用，在ＧＤ附加２．０ｍｇ／ＬＢＡ的培养基上，外植体不定芽诱导率达７０％以上。ＩＡＡ或ＮＡＡ的加入不利于外植体不定芽的诱导。在不定芽生长培养基中，加入适量的ＩＢＡ能促进不定芽的伸长生长。伸长至０．５ｃｍ以上的嫩枝在１／２ＧＤ附加４．０ｍｇ／ＬＩＢＡ的培养基上，生根状况良好。生根后的小植株，在１／２ＧＤ培养基上接种Ｒｔ４９菌根真菌，菌丝侵染进入根部，形成组培菌根植株。
The mature embryos of Pinus massoniana were used as explants to induce
ad-ventitious buds on GD、SH、MS or MCM medium supplemented with 0.5～4.0 mg/LBA
and0.0～1.0 mg/L IAA. It has been showed that the basic medium played a determinative rolein
initiating the adventitious buds. The optimal combination of the medium was GD + 2.0mg/LBA
without IAA,on which over seventy percent of explants was induced.The additionof IAA or NAA
was disadvantageous to the formation of adventitious buds. By adding opti-mal concentration
of IBA in the growth medium,the elongation of the adventitous buds wasimproved.The
elongated shoots were induced to produce adventitious roots on 1/2 GD medi-um
supplemented with 4.0mg/LIB Both the plantlets and mycorrhizal fungi Rt49 wereinoculated on
l/2 GD medium simultaneously. The mycorrhizal fungi infected the root tipsand then
mycorrhizae formed.
出处
《林业科学研究》
CSCD
北大核心
1995年第3期241-246,共6页
Forest Research
基金
"八五"攻关课题
关键词
马尾松
离体培养
菌根
Pinus maassoniana, in vitro ,mycorrhizae
