摘要
本文报道用尼龙网为载体的抗ＨＢｓＡｇ抗体膜作免疫吸咐剂用于酶联免疫测定（ＥＩＡ），对检测ＨＢｓＡｇ的免疫反应程序和反应条件，如酶标／抗原溶液配比量和温育反应时间等进行了研究。结果得到一步温育ＥＩＡ法的检测灵敏度与二步温育ＥＩＡ法相比没有下降，检测时间则由原来的约３ｈ缩短至约５０ｍｉｎ。
Covalent immobilization of antibodtes on nylon net used in solid-phase enzyme-linked im-munoassay is described. The immunological reaction process and optimistical conditions such as the ratio ofenzyme-labeled antibody/antigen and the time of incubation in the detection of HBsAg havebeen reported.Ithas been proved that the sensitlvity of one-step incubation EIA can be compared with that of two-step incuba-tion EIA. The detection time is decreased from about 3h to about 50min.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1995年第3期194-197,共4页
Immunological Journal
基金
天津医科大学青年基金
