摘要
应用高效液相色谱－电化学检测法（ＨＰＬＣ－ＥＣＤ）测定大鼠在注射抗原ＳＲＢＣ后２－７天期间，脾脏和胸腺内多巴胺（ＤＡ）含量的动态变化。实验结果表明，在抗体应答期间，脾脏和胸腺中ＤＡ含量的变化趋势较一致。在免疫应答的初期，ＤＡ含量略高于对照，在应答的高峰日（免疫后第５天）又略低于对照，但都无显著意义。在抗体应答的第６天，脾脏和胸腺中ＤＡ含量均明显高于盐水对照组。以后又很快恢复。上述结果提示，体液免疫应答可影响外周淋巴器官内ＤＡ的代谢，外周ＤＡ可能亦参与免疫功能的调节。
In this study,we determined the changes of content of dopamine(DA) in the spleen and thym us of rats immunized with sheep red blood cells(SRBC) during day 2 to 7 post-immunization by the aid of the high-performance liquid chrom atography with electrochemical detection(HPLC-ECD).The results are as follows:The changing trend of DA content in the spleen was similar to that in the thymus during antibody response.In the early period of immune response,DA content of the immuned rats was slightly higher than that of the control animals,and on the peak day of immune response,DA content of injected-antigen rats was slightly lower than that of the control,but these changes had not marked signiflcance.On day 6 of antibody response,DA content in the spleen and thymus markedly increased compared with the saline control.Afterwards,DA level quickly tended to recover.A bove results suggest that the humoral immune response may influence the metabolism of DA in the peripheral lymphoid organs,and peripheral DA may participate in the modulation of the immune function.
出处
《南通医学院学报》
1995年第1期8-9,共2页
ACTA Academiae Medicinae Nantong
关键词
多巴胺
体液免疫应答
神经免疫调节
dopamine
humoral immune response
neuroimmunomodulation
