摘要
为建立检测人巨细胞病毒（ＨＣＭＶ）感染的ＰＣＲ方法并探讨ＨＣＭＶ与宫颈癌的关系，从石蜡包埋和活检宫颈癌组织中提取ＤＮＡ，用聚合酶链反应体外扩增ＨＣＭＶＥｃｏＲＩＤ片段上一段长１５２ｂｐ的核酸序列，再用地高辛末端转移酶标记法标记和扩增片段互补的ＨＣＭＶ寡核苷酸探针，用该探针证实扩增片段的特异性。检测宫颈癌标本４３例，阳性率为２５．６％（１１／４３），正常宫颈组织８例，阳性率为０。宫颈癌标本阳性率高于正常宫颈标本，提示ＨＣＭＶ与宫颈癌的发生有关。
To establish the PCR method for detection of HCMV and study the relationship between HCMV and cervical cancer, the authors extracted DNA from paraffin-embedded and biopsy samples of cervical cancer, and amplified a 152bp DNA sequence in HCMV EcoRI D fragment by PCR. DIG labeled HCMV oligonucleiotide probe was used to analyse the specificity of the PCR products. As a result, 11 of 43 samples of cervical cancer were positive (25.6%), none of & samples of normal cervix was positive. The result indicated that HCMV was related to cervical cancer.
出处
《山东医科大学学报》
1995年第3期189-192,共4页
Acta Academiae Medicinae Shandong
