摘要
本文以2.5％聚乙二醇(PEG)沉淀伯氏疟原虫感染鼠血清循环免疫复合物(CIC),用化学发光(CL)技术检测疟疾CIC对小鼠腹腔渗出细胞(PEC)产生和释放活性氧的刺激作用。结果表明,疟疾CIC可激发小鼠PEC的CL反应,并可抑制酵母菌激发的CL。本文讨论了激发效应细胞释放活性氧(ROS)与CIC引起免疫病理间的关系。
Circulating immune complexes (CIC) isolated from sera of mice infected with Plasmodium berghei by precipitation with polyethylene glycol were examined for their ability to stimulate the production of reactive oxygen species by peritoneal exudate (PEC) of normal mice, using luminol-aided chemilnminescence (CL). CIC were found to be capable of stimulating the production of CL by normal mouse PEC. Weaker CL reiponses were observed when PEC were incubated with P. berghei soluble antigens. Normal mouse IgG and IgG1, IgG2b subclasses showed no effect on the stimulation of the production of CL. When normal mouse PEC were preincubated with CIC, their CL responses to opsonized zymosan were significantly depressed to 34% of the control level. The data suggest that CIC are capable of stimulating phagocytes to release reactive oxygen species and mediating pathological tissue damage.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1989年第2期85-87,共3页
Chinese Journal of Parasitology and Parasitic Diseases
