摘要
用ＭＳ＋ＢＡ５ｍｇ／Ｌ＋ＩＢＡ０．０１ｍｇ／Ｌ培养基培养柿树品种“黑柿”、“长良”的胚乳愈伤组织获再生植株．再生植株的根尖染色体数目为２ｎ＝９０或１８０，无发现存在２ｎ＝１３５的个体．而作为外植体的胚乳细胞检查为３ｎ＝１３５．表明愈伤组织细胞在培养过程中发生了染色体数量的变化．
Endosperm of Japanese persimmon (Diospyros kaki L. ) cv. 'Nagara', 'Kurogaki' were cultured and some regenerated shoots were obtained from endosperm callus on the MS media with 5. 0mg/L BA and 0. 01mg/L IBA. The chromosome number of the root tip cell was examined. Results indicated that the chromosome numbers in regenerated plants are 2n= 180 or 2n= 90, but not 2n= 135 as had been found in original endosperm cells. It suggested that a change in ploidy had occurred in callus cells during culture.
出处
《汕头大学学报（自然科学版）》
1995年第1期42-47,共6页
Journal of Shantou University：Natural Science Edition
关键词
柿树
胚乳植株
染色体
再生植株
Diospyros kaki, endosperm plant, chromosome number
