摘要
本研究将抗不同表位单抗组合,首次用于夹心斑点-ELISA检测血吸由感染。对慢性日本血吸虫病患者的阳性检出率为85%,12例急性患者均阳性,10例晚血病人4例阳性,非疫区正常人的假阳性率为2.2%。用单盲法检测的-组血清其符合率亦较满意。在基本消灭血吸虫病地区检测了1100人,用夹心斑点-ELISA测抗原,阳性率为6.5%,以IHA测抗体阳性率为25.2%。在低年龄组人群,斑点ELISA与COPT符合率为88.9%,明显高于IHA与COPT的符合率(35.7%),P<0.05。动物实验中,组合单抗夹心班点ELISA对低感染兔的阳性检出率高于单个单抗直接斑点-ELISA。
The study in which McAb against different antigenic determinant were compounded was put into effect for the first time in the sandwich dot-ELISA detection of Schistosoma japonicum infection.The positive rate was 85.0% to chronic schistoso miasis and 100% to 12 acute cases.4 cases showed positive in the detection of 10 advanced schistosomiasis patients and the positive rate was 2.2% to the normal persons.The result was also satisfactory in the blind detection of one batch of serum samples.In the area with schistosomiasis basically eliminated,the positive rate was 6.5% by the means of dot-ELISA,and 25.2% by IHA.In the group of junior years,the coincident rate between dot-ELISA and COPT was 88.9%,which was significantly higher than that between IHA and COPT(35.7%),P<0.05.The positive rate of the detection with compound McAb sandwich dot-ELISA was higher than that of single McAb dot-ELISA(direct method)in the animal experiment of light infection.
出处
《上海免疫学杂志》
CSCD
北大核心
1995年第3期141-144,共4页
Shanghai Journal of Immunology
基金
国家自然科学基金