摘要
在培养的新生大鼠颈上神经节交感神经元标本上，用全细胞电流箝及电压箝技术观察记录了交感神经元膜电学参数及电压门控性通道电流。用全细胞电流箝测得下列平均值：静息电位，－５１±６ｍＶ；输入阻抗，１４３２±３８９ＭΩ；时间常数，１３０±３２ｍｓ；动作电位幅度，９６±１０ｍＶ，超射值，４２±６ｍＶ。培养早期即可记录到自发或诱发的动作电位，大多数动作电位后跟随快或慢的后超极化电位。在电压箝状态下可分别记录到电压门控钠、钾、钙通道电流。钾电流以延迟整流型为主，钙电流只有高电压激活，缓慢失活的Ｌ或Ｎ型，未能记录到低电压激活，快速失活的Ｔ型钙电流。
Na, K and Ca currents and other electrophysiological characteristics of cultured neonatal rat superior cervical sympathetic neurons were studied using whole cell clamp technique. The mean passive and active membrane properties measured are as follows: resting membrane potential, 51±6 mV; input resistance, 1432±389 MΩ; time constant, 130±32 ms; amplitude of action potential, 96±10 mV; overshoot, 42±6mV. Na, K and Ca currents were isolated upon pharmacological manipulations. The predominant type of K current was a noninactivating delayed rectifier. Voltage-clamp studies also showed the presense of a high voltage-activated sustained inward Ca current, while low voltage could not elicit any transient Ca current.
出处
《生理学报》
CAS
CSCD
北大核心
1995年第5期441-447,共7页
Acta Physiologica Sinica
基金
国家自然科学基金
关键词
颈上神经节
交感神经元
膜电学
通道
电压门控
superior cervical ganglion
sympathetic neurons
patch-clamp
sodium current
potssium current
calcium current
