摘要
用抗坏血酸－Ｆｅ（Ⅲ）和过氧化氢分别作用于铜锌超氧化物歧化酶（ＳＯＤ），经疏水层析分离得到亲水型和疏水型ＳＯＤ．用胰蛋白酶和胃蛋白酶分别作用于天然ＳＯＤ，亲水型ＳＯＤ及疏水型ＳＯＤ，结果表明疏水型ＳＯＤ较亲水型ＳＯＤ及天然ＳＯＤ易被降解，提示活性氧氧化修饰后的ＳＯＤ对蛋白水解酶敏感性提高与其疏水性增高有关。
To study the mechanism of the sus-ceptibility changes of cupro-zinc superoxidedismutase(SOD)to proteases induced by re-active oxygen species is the aim of this work.By hydrophobic chromatography,hydrophilicand hydrophobic form of SOD was isolatedfrom SOD modified by hydrogen peroxide orascorbate-Fe(Ⅲ)system.The hydrophobicform of SOD demonstrated a high proteolyticsusceptibility to trypsin and pepsin than boththe native and hydrophilic form of SOD.Theresults suggested that the increased hydropho-bicity of SOD might be responsible for its highsusceptibility to proteases.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1995年第1期47-50,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金
关键词
铜锌
超氧化物歧化酶
活性氧
疏水层析
cupro-zinc superoxide dismutase,reactive oxygen species,hydrophobic chro-matography
