摘要
磁性高分子微球内含磁核，外部是表面带羧基的聚苯乙烯高分子外壳，不仅可以共价偶联酶分子，而且易于磁场分离，是固定化酶的理想载体。采用碳化二亚胺法，在磁性高分子微球上固载ＡＳ１．３９８中性蛋白酶，制备活性达７００Ｕ／ｇ的磁性固定化中性蛋白酶，缓冲液的ｐＨ６．０、离子强度较高时，磁性固定化酶（ＭＩＥ）的活性最高。载体性质对ＭＩＥ活性影响很大，磁性微球粒径愈小，表面羧基含量愈大，ＭＩＥ活性愈高。
A neutral protease from Bacillus subtilis has been immobilized onto a magnetic polystyrene microsphere havig surface carboxyl groups by covalent coupling. The magnetic immobilized neutral protease, having 700U/g enzyme activity, has benn prepared. The magnetic immobilized neutral protease will show optimum activity when a neutral protease is immobilized onto a magnetic microsphere at pH 6. 0 and high ionic strength. With the diameter of magnetic microspheres decreasing and surface carboxyl groups of magnetic microspheres increasing,magnetic immobilized neutral protease activity will increase.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
1995年第3期209-213,共5页
Journal of Biomedical Engineering
基金
国家自然科学基金
关键词
磁性固定化酶
磁性微球
共价
中性蛋白酶
Magnetic immobilized enzyme
Covalent
Neutral protease
Magnetic microphere
