摘要
本文简要介绍了９０年代以来利用ＲＦＬＰ和ＲＡＰＤ分子标记构建林木的遗传连锁图谱，并应用高密度的遗传连锁图谱上的分子标记进行林木重要数量性状的ＱＴＬ作图，进而阐明控制多基因的数目，确定ＱＴＬ在染色体上的位置，测定单个基因的作用效应。遗传连锁图谱构建和数量性状基因定位预示林木遗传育种研究将产生深刻的变革。
Abstract This essay simply describes the construction of genetic maps using RFLP andRAPD markers and introduces the use of markers in mapping the important QTLs on ahigh density linkage map since 90's. Through map constructing and QTLs mapping , wecan reveal the number of genes which control the quantitive trait. ' locate QTLs on chr-omosomes and estimate the effect of single genes. UP to now, map constructing hasbeen done in more than eight tree spieces , such as popular, scots pine , eucalypts ' slashpine, sugar pine, American chestnut ect. Some important QTLs have been mapped onthe corresponding genetic maps ,such as disease resistance, growth , wood quality and soon. Choosing suitable pedigrees is a key step for map constructing and QTL locating.Map constructing in trees is quite different from that in agronomic crops. F2 or back-cross 1 population, backcross 2 population, full-sib cross pedigree and haploid havebeen established respectively according to the feature of different tree spieces, whichmake it possible for such research carried out in forest trees. Although there are somelimitations of their near-term applification , constructing genetic maps and mapping QT-Ls suggest that there will be profound reformation in the study of forest genetics andbreeding.
出处
《世界林业研究》
CSCD
北大核心
1995年第3期6-12,共7页
World Forestry Research
