摘要
用ＬＫＢ平板电泳仪、４．４％聚丙烯酰胺凝胶对南京罗非鱼良种场尼罗罗非鱼、奥利亚罗非鱼养殖群体肌、肝、脑、心、眼中的１０种同工酶进行电泳分析的结果表明，奥利亚罗非鱼的群体未见多态位点，平均杂合度为０，是超“纯”的养殖群体；尼罗罗非鱼群体中有２０％的尼奥杂交鱼，其体形酷似尼罗，难以肉眼鉴别。由此可见，我国尼罗罗非鱼养殖群体中已存在遗传渐渗问题，须注意防杂和提纯。在剔除了杂交鱼后，该场尼罗罗非鱼群体的平均杂合度为０．０４０，ＥＳＴ有多态现象。
With LKB horizontal electrophoresis, 4. 4% polyacrinomide gel, 10enzymes in the muscle, liver, brain, heart and eye of tilapia nilotica (Oreochromis niloticus)and tilapia aurea (Oreochromis aureus) from the Nanjing Tilapia Seed Farm, a national level fish seed farm, were analyzed. No polymorphic locus was found in tilapia aurea, and the average heterozygosity was 0. It indicated that the analyzed tilapia aurea stock is a ″supper pure″ cultivated one. In the tilapia nilotica stock, 20% individuals were found as hybrid between nilotica and aurea, which external appearance closed to the nilotica extremely, and was very difficult to distinguish by eyes. It proves that the introgressive hybridization has happened in hatchery stocks of tilapia in China. Introgression must be prevented by safety keeping of ″pure strain″ and prevention of uncontrolled hybridization. After sorting out the part of hybrids, the heterozygosity of tilapia nilotica reached 0. 040. Enzyme EST showed polymorphism.
出处
《水产学报》
CAS
CSCD
北大核心
1995年第2期105-111,共7页
Journal of Fisheries of China
关键词
尼罗罗非鱼
奥利亚罗非鱼
种群
遗传渐渗
Tilapia nilotica
tilapiaaurea
stock
enzyme
electrophoresis
introgression
