摘要
首次提出了用甲苯破壁提取酵母菌中SOD的方法,此法提取SOD的含量分别为氯仿-乙醇法、酶裂解法和细胞自溶法的1.29、1.08和2.01倍.通过对5种酵母菌的测定,发现酵母菌对氧的抗性与其SOD含量密切相关;同时对一株SOD含量较高的卡尔酵母(Saccharomyces carlsbergensis)BY2菌株进行了营养和培养条件的研究,结果表明它们对该菌株SOD含量和SOD同功酶的影响较大.
A method of breaking cell with toluene has been used for the first timeto extract SOD from yeast, and we have compared this method with other threemethods, we found that SOD activity of crude extraction by this method were respectively 1.29, 1.08 and 2.01 times higher than the methods of treating with chloro-form-ethanol, snailase and cell autolysis. As well as in 5 yeast strains, we discovered that the tolerance of yeast cell to O2 closely related to their content of SOD.The varied culture conditions of one high SOD activity strain (Saccharomyces carl-sbergensis BY2) were studied, the results showed the effects of those conditions onthe content of SOD and SOD isoenzymes.
出处
《微生物学报》
CAS
CSCD
北大核心
1995年第4期280-286,共7页
Acta Microbiologica Sinica
关键词
酵母菌
超氧化物歧化酶
真菌
Yeast, Superoxide dismutase (SOD), Nutrients, Culture conditions
