摘要
以氚标记胸腺嘧啶核苷(3H-TdR)掺入法检测每分钟脉冲数(CPM),比较母牛分枝杆菌活菌悬液、辐射杀死菌悬液及高温杀死菌悬液对健康人外周血T淋巴细胞增殖反应的影响。以此作为评价治疗以细胞介导免疫为主的结核病免疫功能障碍免疫制剂效力的一个重要参数。在加入单一刺激因子实验中,对照组CPM为448±131;加入BCG、母牛分枝杆菌活菌悬液。
This paper present that preparations of M. vaccea, which included suspensions of live bacilli, irradiation-killed bacilli and high temperature killed bacilli, for the effect on lymphocyte proliferative response of peripheral blood in healthypersons were compared by 3H-TdR incorporation method. CPM of control was448±131, CPM adding suspensions of live M. vaccea, irradiation-killed bacilli and hightemperature killed bacilli were 1037±194, 2299±140,1819±528, 994±186,respectively.Stimulative index of all four preparations were more than 2.0, specially suspensionsof live and irradiation killed M. vaccea is more effective than BCG and high temperature killed M. vaccea, which were 5.13, 4.06, respectively. In another experiment, suspensions of live, irradiation-killed and high temperature killed M. vacceaplus rIL-2 were found more effective than rIL-2 alone. CPM of them were 9544±1727, 8530±714, 8230±1035, and 3721±1336, respectively, but BCG was 6904±1218. The results show that preparations of M. vaccea tested have distinctly pro-motive effect for proliferative respinse of lymphocyte in vitro. The addition ofrIL-2 to suspendions of live M. vaccea, irradiation killed and high temperature killed M. vaccea cooperatively increased the effects of proliferative response mentionedabove.
出处
《微生物学报》
CAS
CSCD
北大核心
1995年第4期292-297,共6页
Acta Microbiologica Sinica
关键词
母牛分枝杆菌
微生物制剂
T淋巴细胞
增殖反应
M. vaccea, Immunotherapy, Proliferative response of lymphocyte in vitro, 3H-TdR incorporation method