摘要
从200株菌中筛选出具有双酮化合物还原活力较高的菌株7株,其中以白地霉(Geotrickum, sp. )G38活力最高,而且有较好的区域选择性和立体选择性。白地霉G38能对β-羰基苯丙酸乙酯进行不对称还原,生成(R)-β-羟基苯丙酸乙酯,用于制备抗忧郁药(R)-fluoxetine。用二甲基硅橡胶包埋白地霉菌体取代游离菌体进行生物转化,可以提高反应的立体选择性,对映体过量值从49％提高到81％。
Seven strains of microorganisms which have activity of dicarbonyl compound reduction were isolated, in which Geotrichum sp. G38 showed the highest activity and good regioselectivity and stereoselectivity. Using Geotrichum sp. G38, ethyl benzoylacetate was reduced to (R)-ethyl 3-Phenyl-3-hydroxy propionate which was the intermediate in preparing (R)-fluoxetine. The ee value was improved from 49% to 81% when silicone-immobilized cells was used instead of free cells in biotransformation.
出处
《微生物学报》
CAS
CSCD
北大核心
1995年第5期353-357,共5页
Acta Microbiologica Sinica
基金
中国科学院"八.五"重点应用项目
